Volume 4, Issue 20 (2015)                   JCP 2015, 4(20): 739-746 | Back to browse issues page

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Lotfi A, Shams-Bakhsh M, Yakhchali B. Production of polyclonal antiserum against beet western yellows virus coat protein expressed in Escherichia coli. JCP. 4 (20) :739-746
URL: http://journals.modares.ac.ir/article-3-12048-en.html
1- Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
2- National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Abstract:   (1504 Views)
Serological methods are commonly used methods for detection of viruses. Preparation of pure viral antigens is a crucial step in production of antibodies required for serological studies. In this research the gene encoding coat protein of a Beet western yellows virus (BWYV) isolate from Iran was amplified by PCR and was ligated into a bacterial expression vector (pET26b) to obtain pET-BWYV-CP clone. Escherichia coli BL21 was transformed with pET-BWYV-CP and expression of the recombinant coat protein was induced by IPTG. The expressed recombinant coat proteins were purified and used as an antigen for rabbit immunization. The antiserum was able to detect recombinant coat protein in total protein extracts of induced E. coli BL21 cells in western blot analysis.  
Full-Text [PDF 343 kb]   (1144 Downloads)    
Article Type: Full Paper | Subject: Plant Virology
Received: 2015/02/12 | Accepted: 2015/09/16 | Published: 2015/11/1

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