Department of Biochemistry, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, Maharashtra 431004, India.
Chilo partellus is a devastating sorghumand maize pest that exists worldwide. C. partellus gut proteases (CPGPs) were isolated, purified and characterized. We analyzed potential trypsin and chymotrypsin inhibitors (TIs) from tuber plants to identify the protease inhibitors (PIs) of CPGPs. PIs from Ipomoea batatas completely inhibited protease activity and growth and led to reduced C. partellus insect larvae weights. One PI was purified from I. batatas tubers and identified as a potent antagonist of CPGPs. In conclusion, we identified Ipomoea batatas inhibitors with significant potential applications in developing resistant transgenic sorghum and maize crops.
Baker, J. E. 1977. Substrate specificity in the control of digestive enzymes in larvae of the black carpet beetle. Journal of Insect Physiology, 23: 749-753.
Bhavani, P., Bhattacherjee, C. and Prasad, T. 2007. Bioevaluation of subabul (Leucaena leucocephala) proteinase inhibitors on Helicoverpa armigera. Arthropod- Plant Interaction, 4 (1): 255-261.
Bolter, C. J. and Jongsma, M. A. 1995. Colorado potato Beetles (Leptinotarsa decemlineata) adapt to proteinase inhibitors induced in potatoleaves by methyl jasmonate. Journal of Insect Physiology, 41:1071-1078.
Bown, D. P., Wilkinson, H. S. and Gatehouse J. A. 1997.Differentially regulated inhibitor-sensitive and insensitive protease genes from the phytophagous insect est, Helicoverpa armigera, are members of complex multigene families. Insect Biochemistry and Molecular Biology, 27: 625-638.
Broadway, R. M. 1989. Characterization and ecological implications of midgut proteolytic activity in larval Pieris rapae and Trichoplusia ni. Journal of Chemical Ecology, 15: 2101-2113.
Broadway, R. M. 1995.Are insects resistant to plant proteinase inhibitors? Journal of Insect Physiology, 41:107-116.
Broadway, R. M.1996. Dietary proteinase inhibitors alter complement of midgut proteases. Archives of Insect Biochemistry and Physiology, 32: 39-53.
Broadway, R. M. 1997. Dietary regulation of serine proteinases that is resistant to serine proteinase inhibitors. Journal of Insect Physiology,43: 855-874.
Chandrashekar, A. and Satyanarayana, K. V.2006. Disease and pest resistance in grains of sorghum and millets. Journal of Cereal Science, 44: 287-304.
Davis, B. J. 2006. Disc electrophoresis II, methods and application to human serum, annals of the New York Academy of Science,21: 404-429.
Glas, J. J., Van den Berg, J.and Potting, R. P. J. 2007.Effect of learning on the oviposition preference of field-collected and laboratory-reared Chilo partellus (Lepidoptera: Crambidae) populations. Bulletin of Entomological Research, 97: 415-420.
Gooding, R. H. 1975.Digestive enzymes and their control in Haematophagus arthropods. Acta Tropica, 32: 96-111.
Harsulkar, A. M., Giri, A. P., Patankar, A. G., Gupta, V. S., Sainani, M. N., Ranjeka, P. K. and Deshpande, V. V.1999.Successive use of non-host plant proteinase inhibitors required for effective inhibition of Helicoverpa armigera gut proteinases and larval growth. Plant Physiology, 121: 497-506.
Harsulkar, A. M., Giri, A. P., Gupta, U. S., Sainani, M. N., Deshpande, V. V. and Ranjekar, P. K. 1998. Characterization of Helicoverpa armigera gut proteinases and their interaction with proteinase inhibitors using the Gel X-ray Film Contact Print Technique.Electrophoresis, 19: 1397-1402.
Jongsma, M. A., Stiekema, W. J. and Bosch, D.1996. Combating inhibitor insensitive proteases of insect pests. Trends in Biotechnology, 14: 331-333.
Jouanin, L., Bonade-Bottino, M., Girard, C., Morrot, G. and Gib, M. 1998.Transgenic plants for insect resistance. Plant Science, 131: 1-11.
Kranthi, K.R., Jadhav, D., Wanjari, R., Kranthi, S. and Russell, D. 2001. Pyrethroid resistance and mechanisms of resistance in field strains of Helicoverpa armigera (Lepidoptera: Noctuidae). Journal of Economic Entomology, 1 (94): 253-263.
Kumar, H. 1997. Antibiosis as a resistant mechanism to Chilo partellus (Swinhoe) (Lipidoptera: Pyralidae) in selected maize genotypes. Crop Protection, 16: 331-336.
Laura, V., Jordi, Q., Donaldo, M., Jean, C. B., Victoria, M., Isabel, M., Jean, M. V., Joaquima, M., Emmanuel, G. and Blanca, S. S.2005.Expression of the maize proteinase inhibitor (mpi) gene in rice plants enhances resistance against the striped stem borer (Chilo suppressalis): effects on larval growth and insect gut proteinases. Plant Biotechnology Journal, 3 (2): 187-202.
Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J.1951.Protein measurement with the folin phenol reagent. The Journal of Biological Chemistry, 193:265-275.
Mailafiya, D. M., Le Ru, B. P., Kairu, E. W., Dupas, S. and Calatayud, A. 2011.Asitism of Lepidopterous stem borers in cultivated and natural habitats.Journal of Insect Science, 11-15.
Michaud, D. 1997.Avoiding protease-mediated resistance in herbivorous pests. Trends in Biotechnology, 15: 4-6.
Onyango, F. O. and Oching-Oders, J. P. R. 1994. Continuous rearing of the maize stem borer Busseola fusca on an artificial diet. Entomologia Experimentalis et Applicata, 73: 139-144.
Orr, G. L., Strickland, J. A. and Walsh, T. A. 1994.Inhibition of Diabrotica larval growth by a multicystatin from potato tubers. Journal of Insect Physiology, 40: 893-900.
Panchal, B. B. and Kachole, M. S. 2012. Identification of potent inhibitors of Chilo partellus (Swinhoe) (Lepidoptera: Pyralidae) gut proteinase from plant gum PIs. International Journal of Science Technology, 12 (1): 662-670.
Patankar, A. G., Giri, A. P., Harsulkar, A. M., Sainani, M. N., Deshpande, V. V., Ranjekar, P. K. and Gupta, V. S. 2001.Complexity in specificities and expression of Helicoverpa armigera gut proteases explains polyphagous nature of the insect pest. Insect Biochemistry and Molecular Biology, 31: 453-464.
Pichare, M. M. and Kachole, M. S.1994.Detection of electrophoretically separated proteinase inhibitors using X-ray film. Journal of Biochemical and Biophysical Methods, 28: 215-224.
Ryan, C. A.1990.Proteinase inhibitors in plants: Genes for improving defenses against insect and pathogens. Annual Review of Phytopathology, 28: 425-449.
Shimoto, M. and Chrispeels, M. J. 1996. Protective mechanism of the mexican bean weevil against high levels of α-amylase inhibitor in the common bean. Plant Physiology, 111: 393-401.
Shukla, S., Arora, R. and Sharma, H. C.2005.Biological activity of soybean trypsin inhibitor and plant lectins against cotton bollworm/legume pod borer, Helicoverpa armigera. Plant Biotechnology Journal, 22 (1): 1-6.
Tamhane, V. A., Chougule, N. P., Giri, A. P., Dixit, A. R., Sainani, M. N. and Gupta, V. S. 2005. In vivo and in vitro effect of Capsicum annum proteinase inhibitors on Helicoverpa armigera gut proteinases. Biochimica et Biophysica Acta, 17 (22): 156-167.
Telang, M. A., Giri, A. P., Sainani, M. N. and Gupta, V. S. 2005. Characterization of two midgut proteinases of Helicoverpa armigera and their interaction with proteinase inhibitors. Journal of Insect Physiology, 51 (5): 513-522.
Telang, M., Srinivasan, A., Patankar, A., Harsulkar, A., Joshi, V., Damle, A., Deshpande, V., Sainani, M., Ranjekar, P. K., Gupta, G., Birah, A., Rani, S., Kachole, M., Giri, A. P. and Gupta, V. 2003. Bitter gourd proteinase inhibitors potential growth inhibitors of Helicoverpa armigera and Spodoptera litura. Phytochemistry, 63: 643-652.
Varshney, A. K., Singh, A. K. and Agarwal, H.C. 2007. Orientational responses of Chilo partellus (Lepidoptera: Crambidae) larvae to visual and olfactory stimuli from leaves of two cultivars of maize Zea mays.International Journal of Tropical Insect Science, 27 (01): 15-20.
Yan, C. A. 1990.Protease inhibitors in plants: Genes for improving defense against insects and pathogens. Annual Review Phytopathology, 28: 425-449.