1Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
2Department of Plant Pathology,College of Agriculture,Tarbiat Modares University,Tehran, Iran.
Leptonchus granulosus, recovered from Lorestan province, is described and illustrated based on morphological, morphometric and molecular data. The Iranian population of the species is characterized by its body length of 1091 - 1374 mm, cuticle distinctly two layered, outer layer finely annulated, inner layer distinctly annulated, being partly separated from the body and shriveled after fixation, cap-like lip region separated from the rest of body by constriction, distinctly sclerotised walls of prestoma and stoma, delicate needle-like odontostyle with distinct narrow lumen, 8.0-9.5 mm long, slightly arcuate odontophore, 17-21 mm long, with arms slightly thickened at base, small pear-shaped pharyngeal bulb, occupying 16.6-24.3% of pharynx length, simple intestine, very long prerectum (617-663 µm long), its junction with intestine having three distinct guard cells located between anterior ovary and cardia, didelphic female reproductive system, composed of equally sized less developed tracts, but with distinct parts (tubular uterus, simple oviduct and ovary), conoid to hemispheroid tail and absence of males. In comparison with the available reports of the species, no remarkable variation in morphometric data ranges was observed. This is the first representative of the genus for Iran’s nematode fauna found so far. Molecular phylogenetic studies of Iranian population of L. granulosus using 1669 nt partial sequences of 18S rDNA revealed it forming a clade with another isolate of the species in Bayesian inference (BI) with 0.95 Bayesian posterior probability (BPP).
Andrássy, I. 2009. Free-Living Nematodes of Hungary. III. Pedozoologica Hungarica n. 5. Hungarian NaturalHistory Museum. Budapest, Hungary. 608 pp.
Baqri, Q. H and Jairajpuri, M. S. 1968. On Six New Species of Dorylaimida (Nematoda). Journal of Helminthology, 42 (3-4): 243-256.
Campanella, J. J., Bitincka, L. and Smalley, J. 2003. MatGAT: an application that generates similarity/identity matrices using protein or DNA sequences. BMC Bioinformatics, 4: 29.
Cobb, N. A. 1920. One hundred new nemas (type species of 100 new genera). Contribution to a science of Nematology, 9: 217-343.
De Grisse, A. T. 1969. Redescription ou modifications de quelques technique utilis [a] es dan l'etude des n [a] ematodes phytoparasitaires.
Filipjev, I. N. 1934. The classification of the free-living nematodes and their relation tothe parasitic nematodes.-Smithsonian Miscellaneous Collections, 89: 1963.
Holterman, M., van der Wurff, A., van den Elsen, S., van Megen, H., Bongers, T., Holovachov, O. and Helder, J. 2006. Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades. Molecular Biology and Evolution, 23 (9): 1792-1800.
Huson, D. H. and Scornavacca, C. 2012. Dendroscope 3: an interactive tool for rooted phylogenetic trees and networks. Systematic Biology, doi: 10.1093/sysbio/sys062.
Jairajpuri, M. S. and Ahmad, W. 1992. Dorylaimida. Freeliving, Predaceous and Plant-parasitic Nematodes. Oxford & IBH Publishing Co. Pvt. Ltd. New Delhi, India, 458 pp.
Larget, B. and Simon, D. L. 1999. Markov chain Monte Carlo algorithms for the Bayesian analysis of phylogenetic trees. Molecular Biology and Evolution, 16: 750-759.
Nylander, J. 2004. MrModeltest, version 2. Evolutionary Biology Centre, Uppsala University, Uppsala, Sweden.
Pearse, A. S. 1942. Introduction to Parasitology. Springfield, Ill.: Baltimore, Md. 357 pp.
Pedram, M., Pourjam, E. and Peña-Santiago, R. 2012. Description of Caveonchus ferrisae sp. n. (Dorylaimida: Leptonchidae) from northern Iran, with comments on the taxonomy of the genus. Journal of Nematode Morphology and Systematic. 15 (1): 33-40.
Peralta, M. and Pena-Sañtiago, R. 1996. Nematodes of the order Dorylaimida from Andalucia Oriental, Spain. The families Leptonchidae Thome, 1935 and Aulolaimoididae Jairajpuri, 1964. Fundamental and Applied Nematology, 19, 481-497.
Ronquist, F. and Huelsenbeck, J. P. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19 (12): 1572-1574.
Thorne, G. 1930. Predacious nemas of the genus Nygolaimus and a new genus, Sectonema.-Journal of Agricultural Research of USDA, 41: 445-466.
Thorne, G. 1935. Notes on free-living and plant-parasitic nematodes. II-Higher classification groups of Dorylaimoidea. Proceedings of the helminthological Society of Washington, 2: 96-98.
Thorne, G. 1974. Nematodes of Northern Great Plains. Part II. Dorylaimida in part (Nemata: Adenophorea). South Dakota State University Technical Bulletin 41, 120 pp.
Whitehead, A. and Hemming, J. 1965. A comparison of some quantitative methods of extracting small vermiform nematodes from soil. Annals of applied Biology, 55 (1): 25-38