Department of Plant Protection Aboureyhan Campus, University of Tehran, P. O. Box: 11365/4117, Pakdasht, Tehran, Iran.
The effect of salicylic acid (SA) on induction of resistance against root-knot nematode (Meloidogyne javanica) and the effect of M. javanica to induce biochemical defense responses in tomato (Solanum lycopersicum L.) roots at six-leaf stage were investigated. Meanwhile, the effect of different concentrations of SA on mortality of second stage juveniles of M. javanica was examined. Changes in the activity of cytoplasmic peroxidase (POX), catalase (CAT) and phenylalanine ammonia lyase (PAL) enzymes in the roots of tomato seedlings were measured during seven successive days after inoculation with M. javanica in greenhouse. SA was used as soil drench and leaf spray. The efficiency of treatments were evaluated by measuring diameter of galls, number of galls per plant, number of egg masses per plant, number of eggs per individual egg mass, root and foliage fresh weights. The results showed that use of SA as soil drench and leaf spray significantly reduce diameter of galls 28% and 32%, number of galls per plant 40% and 44%, number of egg masses per plant 45% and 49% and number of eggs per individual egg mass 53% and 55% compared to control (inoculated with nematode only). The activity of the enzymes (POX, CAT and PAL) increased in comparison with plants treated with distilled water. The maximum level of larva mortality was observed at 7 mM SA with no significant difference at concentration of 6 mM. SA caused 21.2% mortality of larvae at concentration of 5 mM.
Abbott, W. S. 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18: 265-267.
Abad, P., Gouzy, J., Aury, J. M., Castagnone-Sereno, P., Danchin, E. G., Deleury, E., Perfus-Barbeoch, L., Anthouard, V., Artiguenave, F. and Blok, V. C. 2008. Genome sequence of the metazoan plant-parasitic nematode Meloidogyne incognita. Nature Biotechnology, 26: 909-915.
Banci, L. 1997. Structural properties of peroxidases. Journal of Biotechnology, 53: 253-263.
Ben Amor, N., Ben Hameda, K., Debeza, A., Grignonb, C. and Abdelly, C. 2005. Physiological and antioxidant responses of the perennial halophyte crithmum maritimum to salinity. Plant Science, 168: 889-899.
Cayrol, J. C., Djian, C. and Pijarowski, L. 1989. Study of the nematicidal properties of the culture filtrate of the nematophagous fungus Paecilomyces lilacinus. Revue de Nématologie, 12: 331-336.
Chen, C., Belanger, R. R., Benhamou, N. and Paulitz, T. C. 2000. Defense enzymes induced in cucumber roots by treatment with plant growth-promoting rhizobacteria (PGPR) and Pythium aphanidermatum. Physiological and Molecular Plant Pathology, 56: 13-23.
Davies, M. J., Hawkins, C. L., Pattison, D. I. and Rees, M. D. 2008. Mammalian heme peroxidases: from molecular mechanisms to health implications. Antioxidants & Redox Signaling, 10: 1199-1234.
Eisenback, J. D. 1985. Diagnostic characters useful in the identification of the four most common species of root-knot nematodes (Meloidogyne spp), In: Sasser, J. N. and Carter, C. C. (Eds.), An advanced treaties onMeloidogyne:Biology and control, Raleigh, North Carolina State University, Graphics. pp. 95-112.
Hammerschmidt, R. 2009. Systemic acquired resistance. Advances in Botanical Research, 51: 173-222.
Hussey, R. and Barker, K. 1973. A comparison of methods of collecting inocula of Meloidogyne spp., including a new technique. Plant Disease Reporter, 57: 1025-1028.
Kato, M. and Shimizu, S. 1987. Chlorophyll metabolism in higher plants. VII. Chlorophyll degradation in senescing tobacco leaves; phenolic-dependent peroxidative degradation. Canadian Journal of Botany, 65: 729-735.
Kesba, H. H. and El-Beltagi, H. S. 2012. Biochemical changes in grape rootstocks resulted from humic acid treatments in relation to nematode infection. Asian Pacific Journal of Tropical Biomedicine, 2: 287-293.
Klessig, D. F. and Malamy, J. 1994. The salicylic acid signal in plants. Plant Molecular Biology, 26: 1439-1458.
Korayem, A. M., El-Bassiouny, H. M. S. and El-Monem, A. A. A., Mohamed, M. M. M. 2012. Physiological and biochemical changes in different sugar beet genotypes infected with root-knot nematode. Acta Physiologiae Plantarum, 34: 1847-1861.
Mariutto, M., Duby, F., Adam, A., Bureau, C., Fauconnier, M. L., Ongena, M., Thonart, P. and Dommes, J. 2011. The elicitation of a systemic resistance by Pseudomonas putida BTP1 in tomato involves the stimulation of two lipoxygenase isoforms. BMC Plant Biology, 11: 29.
Mitchum, M. G., Sukno, S., Wang, X., Shani, Z., Tsabary, G., Shoseyov, O. and Davis, E. L. 2004. The promoter of the Arabidopsis thaliana Cel1 endo‐1, 4‐β glucanase gene is differentially expressed in plant feeding cells induced by root‐knot and cyst nematodes. Molecular Plant Pathology, 5: 175-181.
Molinari, S. 2008. Saliciylic acid as an elicitor of resistance to root knot nematodes in tomato, Acta Horticulturae, 789: 119-126.
Mukherjee, A., Babu, S. P. S. and Mandal, F. B. 2012. Potential of salicylic acid activity derived from stress-induced (water) Tomato against Meloidogyne incognita. Archives of Phytopathology and Plant Protection, 45: 1909-1916.
Odjakova, M. and Hadjiivanova, C. 2001. The complexity of pathogen defense in plants. Bulgarian Journal of Plant Physiology, 27: 101-109.
Oka, Y., Koltai, H., Bar-Eyal, M., Mor, M., Sharon, E., Chet, I. and Spiegel, Y. 2000a. New strategies for the control of plant-parasitic nematodes. Pest Management Science, 56: 983-988.
Oka, Y., Nacar, S., Putievsky, E., Ravid, U., Yaniv, Z. and Spiegel, Y. 2000b. Nematicidal activity of essential oils and their components against the root-knot nematode. Journal of Phytopathology, 90: 710-715.
Oostendorp, M., Kunz, W., Dietrich, B. and Staub, T. 2001. Induced disease resistance in plants by chemicals. European Journal of Plant Pathology, 107: 19-28.
Passardi, F., Cosio, C., Penel, C. and Dunand, C. 2005. Peroxidases have more functions than a Swiss army knife. Plant Cell Reports, 24: 255-265.
Regalado, C., García-Almendárez, B. E. and Duarte-Vázquez, M. A. 2004. Biotechnological applications of peroxidases. Phytochemistry Reviews, 3: 243-256.
Reuveni, R. 1995. Biochemical markers as tools for screening resistance against plant pathogens, In: Reuveni, R. (Eds.), Novel Approaches to Integrated Pest Management, CRC Press, Boca Raton, FL, pp. 21-45.
Ryan, C. A. and Jagendorf, A. 1995. Self defense by plants. Proceedings of the National Academy of Sciences of the United States of America, 92: 4075.
Sahebani, N. and Hadavi, N. 2008. Biological control of the root-knot nematode Meloidogyne javanica by Trichoderma harzianum. Soil Biology and Biochemistry, 40: 2016-2020.
Sahebani, N. and Hadavi, N. 2009. Induction of H2O2 and related enzymes in tomato roots infected with root knot nematode (M. javanica) by several chemical and microbial elicitors. Biocontrol Science and Technology, 19: 301-313.
Schneider, M., Schweizer, P., Meuwly, P. and Métraux, J. 1996. Systemic acquired resistance in plants. International Review of Cytology, 168: 303-340.
Tripathi, R. D. 2006. Plant response to environmental stress. International Book Distributing Company.