Efficacy of salicylic acid and acetylsalicylate in enhancing faba bean resistance against Orobanche crenata parasite

El-Mergawi, Ragab; El-Dabaa, Mahmoud; El-Desoki, Ebrahim

doi:10.48311/jcp.2022.1593

Efficacy of salicylic acid and acetylsalicylate in enhancing faba bean resistance against Orobanche crenata parasite

https://doi.org/10.48311/jcp.2022.1593

Volume 11, Issue 2
June 2022

Pages 253-267

Document Type : Original Research

Authors

R. El-Mergawi

M. El-Dabaa

E. El-Desoki

Botany Department, National Research Centre, Giza, Egypt.

Abstract

Orobanche crenata, an obligate holoparasite, causes severe damage to faba bean grown in the Mediterranean region. A greenhouse experiment was conducted to determine and compare the potential of salicylic acid (SA), and acetylsalicylate (AcSA) used as seed soaking (0.5-1.5 mM, each) or foliar spray (2-6 mM, each) to increase faba bean tolerance to Orobanche crenata infection. Under the challenge of Orobanche infection, the application of SA or AcSA enhanced the growth of faba bean plants by improving their viability, height, and fresh and dry weight. SA and AcSA reduced the growth of parasite tubercles and retarded their development to emerged spike, the most harmful stage. AcSA was more effective than SA in increasing faba bean tolerance to O. crenata infection, and seed soaking showed the greatest effect. At 110 days of sowing, soaking seeds in 0.5 or 1 mM AcSA completely prevented the death of infected plants and increased the weight of plants by 22 and 67%, respectively, and pod weight/plant by 512 and 442%. Moreover, these two treatments greatly reduced Orobanche growth, and complete inhibition of tubercles and emerged spikes occurred by soaking seeds in 0.5 mM AcSA. Orobanche infection greatly increased phenolic content and antioxidant activity in the host tissues, but their levels tended to reduce by all salicylate treatments. The results suggest the great potential of soaking host seeds in AcSA, the inexpensive commercial form of SA, can enhance plant resistance against Orobanche parasite.

Keywords

Vicia faba

salicylate compounds

Orobanche

Tolerance

20.1001.1.22519041.2022.11.2.7.0

Subjects

Weed Science (Herbicides)

References
Abbes, Z., Mkadmi, M., Trabelsi, I., Amri, M., Kharrat, M. 2014. Orobanche foetida control in in faba bean by foliar application of benzothiadiazole (BTH) and salicylic acid. Bulgarian Journal of Agricultural Sciences, 20 (6): 1439-1443. DOI: https://doi.hdl.handle.net/20.500.11766/7535
Ahmed, I., Ahmad, T. K. A., Basra, S. M. A., Hasnain, Z., Ali, A. 2012. Effect of seed priming with ascorbic acid, salicylic acid and hydrogen peroxide on emergence, vigor and antioxidant activities of maize. African Journal of Biotechnology, 11: 1127–1137. DOI: https://doi.org/10.5897/AJB11.2266
AL-Wakeel, S. A. M., Moubasher, H., Gabr, M. M. A., Madany, M. M. Y. 2013. Induced systemic resistance: an innovative control method to manage branched broomrape (Orobanche ramosa L.) in tomato. IUFS Journal of Biology, 72: 137–151.
Brand-Williams, W., Cuvelier, M. E. E., Berset, C. L. W. T. 1995. Use of free radical method to evaluate antioxidant activity. LWT- Food Science and Technology, 28: 25-30. DOI: http://dx.doi.org/10.1016/S0023-6438(95)80008-5
Briache, F. Z., Ennami, M., Mbasani-Mansi, J., Lozzi, A., Abdelhadi, A., El Rodeny, W., Amri, M., Triqui, Z. A., Mentag, R. 2020. Effects of salicylic acid and indole acetic acid exogenous applications on induction of faba bean resistance against Orobanche crenata. Plant Pathology Journal, 36(5): 476-490. DOI: https://doi.10.5423/PPJ.OA.03.2020.0056
Brun, G., Braem, L., Thoiron, S., Gevaert, K., Goormachtig, S., Delavault, P. 2017. Seed germination in parasitic plants: what insights can we expect from strigolactone research?. Journal of Experimental Botany, 20: 794–798.
El-Dabaa, M. A. T., Haggag, K. H. E., El-Mergawi, R. A. 2021. Application of Trichoderma Spp. in controlling Orobanche ramosa parasitism in chamomile. Middle East Journal of Applied Sciences, 11(1): 360-367. DOI: https://doi. 10.36632/mejas/2021.11.1.29
El-Mergawi, R. A., Abd El-Wahed, M. S. A. 2020. Effect of high salicylate concentrations on growth and levels of aromatic compounds in six plant species. Asian Journal of plant Sciences, 19(4): 383-389. DOI: https://doi.10.3923/ajps.2020.383.389
El-Mergawi, R. A., Taie, H. A. 2014. Phenolic composition and antioxidant activity of raw seeds, green seeds and sprouts of ten faba bean (Vicia faba) cultivars consumed in Egypt. International Journal of Pharma and Bio Science, 5(2): 609 – 617.
Goldwasser, Y., Hershenhorn, J., Plakhine, D., Kleifeld, Y., Rubin, B. 1999. Biochemical factors involved in vetch resistance to Orobanche aegyptiaca. Physiology Molecular Plant Pathology, 54: 87-96. DOI: https://doi.org/10.1006/pmpp.1998.0191
Gomez, K. A., Gomez, A.A. 1984. Statistical procedures for agricultural research. John Wiley & Sons Inc., Singapore.
Hayat, Q., Hayat, S., Irfan, M., Ahmad, A. 2009. Effect of exogenous salicylic acid under changing environment: A review. Environmental and Experimental Botany, 68:14-25. DOI: https://doi.org/10.1016/j.envexpbot.2009.08.005
Kabiri, R., Naghizadeh, M. 2015. Exogenous acetylsalicylic acid stimulates’ physiological changes to improve growth, yield and yield components of barley under water stress condition. Journal Plant Physiology & Breeding, 5(1): 35-45. DOI: https://iranjournals.nlai.ir/handle/123456789/412543
Kusumoto, D., Goldwaser, Y., Xie, X., Yoneyama, K., Takeuchi, Y., Yoneyama, K. 2007. Resistance of red clover (Trifolium pratense) to the root parasitic plant Orobanche minor is activated by salicylate but not jasmonate. Annals of Botany, 100: 537-544. DOI: https://doi.10.1093/aob/mcm148
Madany, M. Y., Zinta, G., Abuelsoud, W., Hozzein, W. N., Sami, S., Asard, H., Abdelgawad, H. 2020. Hormonal seed-priming improves tomato resistance against broomrape infection. Journal of Plant Physiology, 250: 153184. DOI: https://doi.10.1016/j.jplph.2020.153184
Matysiak, K., Siatkowski, I., Kierzek, R., Kowalska, J., Krawczyk, R. 2020. Effect of foliar applied acetylsalicilic acid on wheat (Triticum aestivum L.) under field conditions. Agronomy, 10(12): 191. DOI: https://doi.org/10.3390/agronomy10121918
Ozpinar, H., Dag, S., Yigit, E. 2017. Allelopathic effects of benzoic acid, salicylic acid and leaf extract of Persica vulgaris Mill. (Rosaceae). South African Journal of Botany, 108: 102–109. DOI: https://doi.org/10.1016/j.sajb.2016.10.009
Pe´rez-de-Luque, A., Jorrı´n, J. V., Rubiales, D. 2004. Crenate broomrape control in pea by foliar application of benzothiadiazole (BTH). Phytoparasitica, 32: 21–29. DOI: https://doi.10.1007/BF02980855
Rubiales, D. 2010. Faba beans in sustainable agriculture. Field Crops Research, 115: 201-202.DOI: http://www.sciencedirect.com/science/journal/03784290
Sauerborn, J., Buschmann, H., Ghiasvan-Ghiasi, K., Kogel, K. H. 2002. Benzothiazole activates resistance in sunflower (Helianthus annuus) to the root-parasitic weed Orobanche cumana. Phytopathology, 92: 59-64. DOI: https://doi: 10.1094/PHYTO.2002.92.1.59.
Sillero, J. C., Rojas-Molina, M. M., Avila, C. M., Rubiales, D. 2012. Induction of systemic acquired resistance against rust, ascochyta blight and broomrape in faba bean by exogenous application of salicylic acid and benzothiadiazole. Crop Protection, 34: 65–69. DOI: https://doi.org/10.1016/j.cropro.2011.12.001
Sima, G., Fatemeh, Z., Naghashi, V. 2012. Determination of peroxidase activity, total phenolic and flavonoid compounds due to lead toxicity in Medicago sativa L. Advances Environmental Biology, 6(8): 2357-2364.
Stalikas, C. D. 2007. Extraction, separation, and detection methods for phenolic acids and flavonoids. Journal of Separation Science, 30: 3268- 3295. DOI: https://doi:10.1002/jssc.200700261
Triki, E., Trabelsi, I., Amri, M., Nefzi, F., Kharrat, M., Abbes, Z. 2018. Effect of benzothiadiazole and salicylic acid resistance inducers on Orobanche foetida infestation in Vicia faba. Tunisian Journal of Plant Protection, 13 (1): 113-125. DOI: https://hdl.handle.net/20.500.11766/8999
Veronesi, C., Delavault, P., Simier, P. 2009. Acibenzolar-S-methyl induces resistance in oilseed rape (Brassica napus L) against branched broomrape (Orobanche ramosa L). Crop Protection, 28: 104-108. DOI: https://doi: 10.1016/j.cropro.2008.08.014
Wani, A. B., Chadar, H., Wani, A. H., Singh, S., Upadhyay, N. 2017. Salicylic acid to decrease plant stress. Environmental Chemical Letters, 15:101–123. DOI: https://doi.org/10.1007/s10311-016-0584-0
Xi, D., Li, X., Gao, L., Zhang, Z., Zhu, Y., Zhu, H. 2021. Application of exogenous salicylic acid reduces disease severity of Plasmodiophora brassicae in pakchoi (Brassica campestris ssp. chinensis Makino). Plos One, 16(6): e0248648. DOI: https://doi.org/10.1371/journal.pone.0248648
Yang, C., Hu, L.Y., Ali, B., Islam, F., Bai, Q. J., Yun, X. P., Yoneyama, K., Zhou, W. J. 2016. Seed treatment with salicylic acid invokes defence mechanism of Helianthus annuus against Orobanche Cumana. Annals of Applied Biology, 169: 408–422. DOI: https://doi.org/10.1111/aab.12311
Yang, B., Rahman, M. H., Liang, Y., Shah, S., Kay, N. 2010. Characterization of defense signaling pathways of Brassica napus and Brassica carinata in response to Sclerotinia sclerotiorum challenge. Plant Molecular Biology Reporter, 28: 253–263. DOI: https://doi.:10.1007/s11105-009-0149-5

XML

PDF 612.93 K